Watching conformational- and photodynamics of single fluorescent proteins in solution

  • Posted on: 10 July 2014
  • By: admin
TitleWatching conformational- and photodynamics of single fluorescent proteins in solution
Publication TypeJournal Article
Year of Publication2010
AuthorsGoldsmith R.H, Moerner W.E
JournalNature Chemistry
Volume2
Pagination179-186
Date PublishedMar
ISBN Number1755-4330
Accession NumberISI:000274648800012
Keywordsallophycocyanin trimers, bile-pigments, brownian-motion, dynamics, energy-transfer, fluctuations, molecule fluorescence, photochemistry, spectroscopy, state relaxation
Abstract

Observing the dynamics of single biomolecules over prolonged time periods is difficult to achieve without significantly altering the molecule through immobilization. It can, however, be accomplished using the anti-Brownian electrokinetic trap, which allows extended investigation of solution-phase biomolecules-without immobilization-through real-time electrokinetic feedback. Here we apply the trap to study an important photosynthetic antenna protein, allophycocyanin. The technique allows the observation of single molecules of solution-phase allophycocyanin for more than one second. We observe a complex relationship between fluorescence intensity and lifetime that cannot be explained by simple static spontaneous emission lifetime, which is typically assumed to be constant. Our methods provide a new window into the dynamics of fluorescent proteins and the observations are relevant for the interpretation of in vivo single-molecule imaging experiments, bacterial photosynthetic regulation and biomaterials for solar energy harvesting.

Alternate JournalNat Chem